We use multidisciplinary approaches to study single-cell, spatio-temporal Signaling Dynamics that regulate Cell Morphogenesis and Fate Decisions.
We study the spatio-temporal signaling events that regulate Cell Morphogenesis and Fate Decisions. The main hypothesis relevant to our research is that these signaling events are highly dynamic, are precisely regulated in time and space, and can be highly heterogeneous within distinct cells of a population. An important current limitation in our field is that this spatio-temporal resolution of signaling is missed in classic biochemical methods that average populations of thousands of cells, or that rely on the analysis of static, steady-states. To address these challenges, we devise novel quantitative approaches to measure/manipulate signaling dynamics at biologically relevant time/length scales.
We combine single-cell live biosensor imaging, dynamic perturbations using microfluidics, and mathematical modelling to dissect how different growth factors control single-cell MAPK ERK dynamics to specify fate decisions.
We have been awarded a highly competitive Human Frontiers Science Program grant to study single cells Signaling Dynamics that regulate Stem Cell Renewal and Neuronal Fate decisions.
This is together with:
- Rafael Carazo-Salas at University of Bristol (https://research-information.bristol.ac.uk/en/persons/rafael-e-carazo-salas(a7638b29-53e4-49ba-82b5-98b21d82f41f).html)
- Andrew Cohen at Drexel University (https://drexel.edu/engineering/about/faculty-staff/C/cohen-andrew/)
Please check out our new accepted manuscript on automated neuronal growth cone segmentation in collaboration with the Danuser lab at UT Southwestern.
Congrats Maria, Ludo and Gaudenz …
We have been awarded a prestigious SNF Sinergia program grant to study feedback regulation from the cytoskeleton to Rho GTPase signaling. This work will take advantage of state-of-the-art live imaging techniques including biosensor imaging, non-neuronal optogenetics and chemical biology techniques.
This multidisciplinary work will be performed in collaboration with the labs of:
- Damian Brunner at UniZH (https://www.imls.uzh.ch/en/research/brunnerd.html)
- Daniel Riveline at IGBMC Strasbourg (http://www.igbmc.fr/riveline/),
- Karsten Kruse at UniGE (https://www.unige.ch/sciences/biochimie/labs/karsten-kruse/)